Please join the Biostatistics Seminar October 22, 2018 at 12:00 Noon at 60 College Street, Rm. 216. Please also refer to the flyer attached.
Speaker: Leying Guan
Institution: Stanford University
Time & Place: 12:00 Noon in Room 216, 60 College Street
Title: “Transcription factor target identiﬁcation via knock-down experiments”
The perturbation of a transcription factor should aﬀect the expression levels of its direct targets. However, not all genes showing changes in expression are direct targets. To increase the chance of detecting direct targets, we propose two new methods. The ﬁrst one is a modiﬁed two-group model using data from a single knock-down experiment. The null group corresponds to genes that are not direct targets, but can have small non-zero eﬀects. The second method constructs a depth constrained network using data from multiple knock-down experiments. I will give details of both methods and present some simulated as well as real data examples.